Method for the Simultaneous Determination of Blood Group and Platelet Antigen Genotypes

ABSTRACT

RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). We developed a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform to genotype blood for a plurality of blood group antigen-associated SNPs, including but not limited to: RhD (2), RhC/c, RhE/e, S/s, K/k, Kp a/b , Fya/b, FYO, Jk a/b , Di a/b , and HPA-1a/b.

TECHNICAL FIELD

This invention relates to an ultra high throughput (UHT) multiplex PCR genotyping method. More specifically, the present invention relates to an automated method of determining a plurality of blood group and platelet antigen, preferably human platelet antigen (HPA), genotypes simultaneously from a single sample through the detection of single nucleotide polymorphisms (SNPs) for various blood group and platelet antigens.

BACKGROUND OF THE INVENTION

At present, there are 29 blood group systems and 6 HPA systems recognized by the International Society of Blood Transfusion (ISBT), wherein, with a few exceptions, a blood group ‘system’ may be defined by a single gene at a given locus of the human genome (Daniels, G. L. et al. Vox Sang 2003; 84:244; Metcalfe P. et al., Vox Sang. 2003; 85:240). Most people know their ABO and Rh blood group. However, the ABO and Rh blood group systems expressed on red cells simply represent antigens from only two of the 29 blood group systems, and more systems are being discovered each year. Some examples of blood group systems are the ABO, Rh (D, C, c, E, e), P, Lutheran, Kell (K, k), Lewis, Duffy (Fy^(a), Fy^(b)), or Kidd (Jk^(a), Jk^(b)). Moreover, there are over 250 blood group and 12 human platelet antigens assigned to one of the blood group or HPA systems, respectively. A system is defined by a gene or group of genes at a specific locus of the human genome. The alleles or genotype of a person for each blood group or HPA system represent the unique nucleotide gene sequences that express specific blood group or platelet antigens (for a review see Denomme, G. et al., Approaches to Blood Group Molecular Genotyping and Its Applications: in Stowell, C. and Dzik W., editors; Emerging Technologies in Transfusion Medicine, AABB 2003, Ch 4).

A blood group or HPA system maps to a specific region of the human genome, termed a locus. Nearly all blood group or HPAs can be identified by the presence of its unique nucleotide sequence, termed an ‘allele’, at the locus of interest. Every person has two alleles for any given autosomal gene. Some individuals are homozygotes for a specific allele, i.e. they have two identical alleles, while others are heterozygotes for a specific allele, i.e. they have two different alleles. By definition, alleles that represent different blood group or HPAs differ by at least one nucleotide; sometimes they differ by several nucleotides. For example, a deoxythymidine (T) or a deoxycytidine (C) nucleotide can be found at cDNA position 196 of the glycoprotein IIIa (GP3A) gene that expresses the HPA-1 (Newman P. J. et al., J Clin Invest 1989; 83:1778). The allele containing the deoxthymidine nucleotide expresses the HPA-1a antigen and the allele containing the deoxycytidine nucleotide expresses the HPA-1b antigen. We refer to the T/C nucleotide difference between the two alleles as a single nucleotide polymorphism (SNP).

Blood group alleles for a given blood group system represent genetic variations of the same gene. For example, the ABO blood group system has 3 common alleles, that confer 6 genotypes within this blood group system. Moreover, many alleles within a blood group system express different blood group ‘antigens’, that is to say, dependent on the allelic genotype the corresponding antigenic phenotype is accordingly expressed. Alleles differ in their nucleotide sequence, and the difference between one allele and another, usually within a single blood group system, may be one single nucleotide variation. Therefore, two alleles can differ by one nucleotide, i.e. a SNP and represent a co-dominant bi-allelic system. Alternatively, alleles can differ by a few to several dispersed nucleotides, or by a stretch of nucleotides, any one of which can be used to identify the alleles. Regardless of whether the variations in the nucleotide are due to single or multiple nucleotide differences, the phenotype associated with a specific genotype (the specific nucleotide sequence) will result in the expression of a specific blood group or platelet antigen on the red cell or platelet surface, respectively.

Normally, all blood donations are blood grouped for ABO and RhD. However, sometimes a previously transfused recipient will require more blood that is antigen-matched with one of their own antigens because they have made antibodies to a different blood group or platelet antigen. The gold standard in the industry is to ‘phenotype’ blood for the presence of specific blood group and platelet antigens using government regulated antisera (antibodies) performed by single-test methods or by an automated platform, which is a cost ineffective method for a blood collection facility that routinely performs tests on a high volume basis.

Blood group phenotypes are presently determined using commercially available government-regulated serological reagents and human red cells. These known tests rely on the principle of antibody binding and red cell agglutination to identify clinically important blood group phenotypes. The presently known tests were originally devised some 60 years ago and today require the use of government regulated (for example, Health Canada) approved serological reagents. Some of the tests being employed today have been automated (for example, ABO and Rh typing) while some have been semi-automated (for example, RhC/c and RhE/e). However, many of the presently used tests are performed manually by highly-trained laboratory technologists and are done on a test-by-test basis. In other words, a technologist must perform four separate tests to determine, for example, the Fy^(a), Fy^(b), Jk^(a) and Jk^(b) phenotype of a single blood donation. Essentially, the current tests which employ government-approved reagents in a manual, single-test driven method are a very cost ineffective method for a blood collection facility that is often required to perform such tests on a high volume basis.

In an effort to reduce costs, a blood collection facility will often use non-regulated antisera to ‘screen’ blood donations for important blood group phenotypes and then confirm the phenotype with the regulated antisera. However, since much of the blood is sent to hospitals within 24-48 hours after collection, manual blood group phenotyping cannot meet the short turn-around time required to provide the end user with the information required before blood must be shipped. Therefore, hospital blood banks must perform their own tests on the blood that they have in their inventories. It would be advantageous to provide a cost effective blood screening method that would provide quick and reliable results relating to the clinically important blood group phenotypes.

The prior art uses two basic techniques to detect SNPs; polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (Chaudhuri A., et al. 1995; 85:615), and sequence specific primer (SSP)-PCR (McFarland J. G. et al., Blood 1991; 78:2276). For PCR-RFLP analysis, restriction enzymes are used to digest PCR amplified genomic DNA fragments. In brief, DNA is extracted from nucleated blood cells manually for each blood sample to be analyzed. The PCR is set up manually; a separate PCR is performed on each sample for each SNP of interest. The PCR amplified fragments are digested with a specific restriction enzyme and the digested products are separated on a gel. The pattern of digested DNA fragments viewed from the gel predicts the presence or absence of either nucleotide of a SNP of interest. In SSP-PCR, two PCRs are set up in separate tubes for each SNP of interest. One tube contains a universal primer and a primer with a sequence that is specific to detect one nucleotide of a SNP. The other tube contains the same universal primer and a primer specific for the other nucleotide of a SNP. Prior art has used two pair or three pair PCR to analyze a nucleotide for a given SNP, with at least one pair acting as an internal control to ensure DNA is available for PCR amplification. The prior art does not provide the use of multiple DNA sequences as primer pairs that work simultaneously on a single sample. Moreover, the prior art does not employ novel DNA sequences to detect blood group SNPs in an automated high-throughput fashion.

St-Louis M., et al. (Transfusion 2003; 43:11126-32) have used allele-specific PCR-ELISA to detect blood group SNPs, wherein some of the PCR primers were publicly known and all primers were labeled with digoxigenin; SNPs were detected by oligonucleotide hybridization using solid-phase microplate wells coated with individual blood group-specific complementary oligonucleotides. An abstract by Buffleir E. et al. (Transfusion 2003; 43:92 A) outlines a combined HPA-1 and HPA-5 genotyping method that uses biotin labeled PCR-amplified targets and allele specific oligonucleotide probes arrayed on the bottom of 96 well microplates. Specific hybridization is detected with the use of an enzyme conjugate which produces a specific colourimetric signal. An array of several oligonucleotides reportedly can be used to detect HPA SNPs. The publications, cited above, do not use multiplex PCR primers, nor do they use extension probes, and rely on a less sensitive and more error-prone allele-specific hybridization to detect the SNPs. There are a few other publications that refer to the multiplex PCR amplification of the RHD gene alone, or together with sex determination, or with internal control primers designed to confirm the presence of DNA in various blood group PCR applications. U.S. Pat. No. 5,723,293 describes a diagnostic method and kit for determining Rh blood group genotypes, wherein there is provided a method for directly determining D and associated CcEe genotypes using restriction fragment length polymorphisms (RFLPs) for diagnosis. U.S. Pat. No. 5,804,379 describes a diagnostic method and kit for determining Kell blood group genotype, wherein there is provided a method for determining the K1/K2 genotype using RFLPs for diagnosis. U.S. Pat. No. 5,780,229 provides polynucleotides for determining the Pen polymorphism of human platelet membrane glycoprotein IIIa, and generally describes diagnostic and therapeutic uses relating to the “Pen” human platelet polymorphism (HPA-4) and differs from the teachings of the present invention. United States patent application 20020098528 describes methods and apparatus for blood typing with optical bio-disc, and essentially describes a method for determining the ABO blood cell type of an individual with optical bio-discs and a disc-reading apparatus.

In the SSP-PCR application by St. Louis et al. (Transfusion 2003; 43:1126), two PCR primer pairs are set up, each in a separate well, to detect the nucleotides of a SNP of interest. For example, one primer pair containing a universal primer and a sequence specific primer is set up in a tube to detect a nucleotide of a SNP. Another primer pair containing the same universal and another sequence specific primer is set up in another tube to detect the alternate nucleotide for the same SNP. In addition, each tube includes a primer pair that detects a universal sequence contained in all human DNA. Contained in the PCR tube is digoxigenin-dUTP that is incorporated into the amplified DNA fragment if the sequence specific primer detects the appropriate nucleotide of an SNP. For the detection phase, one of each primer pair contains the chemical tag biotin, which is used to capture the DNA amplified fragment in sets of microtitre wells containing streptavidin. An optical colorimetric assay is used to detect the presence of digoxigenin-dUTP in each of the wells; anti-digoxigenin peroxidase conjugated antibody detects the presence of digoxigenin dUTP and the peroxidase can convert a substrate added to the well into a colored end product. Therefore, the presence of a nucleotide of a SNP is detected by the presence of a color in the microtitre well. Such assays are routinely designed in a 96-well microtitre plate format to facilitate semi-automation. The calorimetric results are evaluated by the operator to determine the presence or absence of the nucleotides for a SNP. The deficiencies of these test systems are the use of a single PCR reaction for each nucleotide of a given nucleotide of each SNP, and the pooling of samples prior to the detection phase and manual post-analyte data analysis.

No prior art has used a multiple, or 12, primer pair multiplexed PCR that successfully works in a single tube, nor has prior art employed novel DNA sequences as probes to detect both nucleotides of a plurality of blood group and HPA genotypes simultaneously, such as the detection of all 12 blood group and HPA SNPs in these mixtures using an automated high-throughput platform.

Accordingly, there is a need for a high-throughput automated multiple blood-group associated SNP analysis of genomic DNA that is capable of rapidly and accurately determining the genotypes and associated phenotypes of a plurality of blood group systems in a single test sample.

SUMMARY OF THE INVENTION

The present invention provides a method of detecting the presence or absence of nucleotides relating to various SNPs for the determination of a specific genotype and accordingly the inferred phenotype. More specifically, the present invention allows for the detection of the presence or absence of two nucleotides of a plurality of different SNPs, and more preferably of the 12 SNPs in a preferred embodiment of the present invention.

The present invention accordingly provides an automated, or robotic, high-throughput ‘screening’ tool for blood group and platelet antigens by evaluating the alleles of the genes that express these antigens on red cells and platelets, respectively. This is done by identifying the unique nucleotides associated with the specific alleles that occupy the gene locus using a testing platform, which requires novel and specific compounds that we designed. Our robotic high-throughput platform provides important blood group and HPA genotype information within 24 hours from the start of the test. We identified the alleles of blood group antigens for; RhD, RhC, Rhc, RhE, Rhe, S, s, Duffy (Fy)^(a), Fy^(b), K, k, Kp^(a), Kp^(b), Diego (Di)^(a), Di^(b), Kidd (Jk)^(a), Jk^(b), and the platelet antigens, Human Platelet Antigen (HPA)-1a and HPA-1b, representing, but not limited to 19 of the most clinically important antigens in red cell and platelet transfusion. Additional genotyping tests for other clinically important blood group and platelet antigens may be developed, and are encompassed in the teachings of the present invention. When performed on all blood donations for all clinically important blood group and platelet antigens, our invention will provide a comprehensive database to select and confirm the antigens when required using government regulated antisera. The use of this platform as a screening tool will lessen the number of costly government regulated tests to be done by the collection facility and end user (the hospital blood bank), and meet the demand of antigen-matched blood for specific transfusion recipients.

The invention discloses a method for DNA-based blood group genotyping for clinically important blood group and platelet antigens. The technology uses an ultra high-throughput multiplex PCR design to detect specific SNPs that represent clinically important blood group antigens: RhD, RhC, Rhc, RhE, Rhe, S, s, Duffy (Fy)^(a), Fy^(b), K, k, Kp^(a), Kp^(b), Diego (Di)^(a), Di^(b), Kidd (Jk)^(a), Jk^(b), and the platelet antigens, Human Platelet Antigen (HPA)-1a and HPA-1b. It should be noted however that the present invention is not limited to the detection of SNPs for only the SNPs listed, but additionally comprises the detection of SNPs for all blood group and platelet antigens. The invention discloses novel DNA sequences of PCR primers that are specifically designed to avoid inter-primer pair cross-reactions and post-PCR probes that make multiple analyses possible. The invention represents a novel approach to screening multiple blood group and HPA genotypes at once and addresses a clear need in the art for novel, rapid, cost-effective and reliable genotyping. This additionally replaces the use of expensive and difficult-to-obtain serological reagents, which can be reserved for use to confirm only the donors identified by the screening process.

More specifically, the present invention analyzes the HPA-1 GP3A mutation incorporated into our SNP assay, and the other blood group antigen SNPs in a method according to the present invention.

The invention addresses the need for an automated, accurate, rapid and cost-effective approach to the identification of multiple blood group antigens. The multiplex SNP assay design and automated genotyping platform allows one trained research technician to identify a plurality of blood group alleles, and more specifically, 19 blood group alleles, overnight on 372 to 2232 individual blood samples. In one application of the present invention, the multiplex PCR and SNP detection platform analyzed the nucleotides of 12 SNPs overnight on 372 individual blood samples. The cost using current standard blood group serology for 372 samples is estimated at CDN$99,500, which reflects a reagent cost of CDN$54,000 (excluding new capital equipment investments) and an operator cost of CDN$45,500 to analyse each of the antigens by Gel Card technology (n=5), immediate spin tube test (n=2), indirect antiglobulin tube test (n=8), and platelet GTI® test (n=1). Approximate 10 to 15 fold cost savings are obtained in the simultaneous DNA-based determination of these blood group alleles. It should be noted that the present invention is not limited to the detection of only 12 SNPs, and may be optimally used for the detection a plurality of SNPs for potentially all blood group and platelet alleles. Accordingly, the products, methods, platform and teachings of the present invention can detect all blood group and HPA SNP variations on a great number of samples, such as 744 samples overnight, as further described below.

The present invention overcomes the deficiencies of the prior art because the entire test, i.e. all steps of the method of the present invention, from PCR to computation analyses can be automated and multiplexed so that the nucleotides of a plurality of SNPs, and more preferably, the 12 SNPs of the present invention, can be identified simultaneously. This automated multiplex high throughput analysis can meet the demand of testing hundreds of blood samples, and the turn-around time of less than 24 hours, to provide valuable information to a blood collection facility before blood is shipped to the end user. This platform has the advantage over existing technology in that it reduces operator handling error. In addition, there are significant cost reductions compared with the current government-regulated serological analysis. It should be noted that present prior art technologies relating to PCR-RFLP and SSP-PCR for blood group and platelet antigens are not routinely used since they are no more cost efficient than serology. The present invention overcomes the deficiencies of the prior art and fulfils an important need in the present art for the automated, accurate, rapid and cost-effective identification of multiple blood group and HPA SNPs.

The invention provides the opportunity to screen all blood donors to obtain a daily or ‘live’ repository of the genotypes or combinations of genotypes currently available for specific transfusion needs. Accordingly, the present invention fulfills a need relating to the collection and antigen screening of blood and blood products.

For convenience, some terms employed in the present specification are noted below. Unless defined otherwise, all technical and scientific terms used herein have the meanings commonly understood by one of ordinary skill in the present art.

The present invention provides a method or screening assay for the determination of blood genotypes of the various blood group and HPA systems through the ultra high throughput multiplex PCR analysis of SNPs in an automated platform (Petrick J. Vox Sang 2001; 80:1). A platform, as referred to herein, refers to a system of machine(s) and protocol(s) capable of analyzing multiplex PCR amplified SNPs, wherein said platform is not limited to, but may comprise the GenomeLab SNPStream (Beckman Coulter Inc., Fullerton, Calif.), the SNPStream™ UHT (Orchid BioSciences, Princeton, N.J.), the SNPStream™ 25K (Orchid BioSciences, Princeton, N.J.), the MALDI-TOF/Mass-Spectrophotometer Spectro CHIP (Sequenom, San Diego, Calif.), and the Gene Chip Microarray (Affymetrix, Inc., Santa Clara, Calif.), Nano Chip (Nanogen, San Diego, Calif.) and the Random Ordered Bead Arrays (Illumina, Inc., San Diego, Calif.) or any other system, machine or protocol capable of analyzing multiplex PCR amplified SNPs. Accordingly, the present invention provides a platform, or system and protocols, for the evaluation and detection of SNPs, for the purpose of typing (determining the genotype and corresponding phenotype) blood group and platelet, preferably, human platelet antigen (HPA) SNP analysis. A preferred platform that can be used in accordance with the present invention is the Orchid SNP-IT system for HLA typing (Orchid Bioscience, Princeton, N.J.), wherein a preferred embodiment of the present invention comprises the use of the primer pairs of Table 1 for the specific oligonucleotide primer extension of blood group and platelet, preferably, human platelet antigen (HPA) SNPs, and the probes of Table 2 for the specific hybridization thereof, and the simultaneous analysis of the absence or presence of a plurality of blood group and platelet, preferably, human platelet antigen (HPA) SNPs using a platform as described herein, or using any SNP analysis system capable of detecting multiplex PCR amplified SNPs.

For the purposes of the present disclosure, SNPs, may refer to any blood group and HPA SNPs, and more preferably refers to any of the SNPs specified in Table 1, or any other known blood group or HPA SNPs or single nucleotide changes including, but not limited to, nucleotide substitutions, deletions, insertions or inversions, that can be defined as a blood group or HPA SNP due to nucleotide differences at the specified position in a gene sequence.

Ultra high throughput (UHT) refers to the implementation of the platform in a rapid and optimized form, that is to say, through the analysis of multiple SNPs. That is to say, UHT analysis refers to the rapid and simultaneous evaluation of a plurality of samples for a plurality of markers, in this case SNPs. For example, the analysis of 12 SNPs (equivalent to 12 C and 12 T nucleotides) for 372 samples, would result in the generation of 8928 (i.e. 2×12×372) determinations that are analysed, an evaluation that far exceeds the number of evaluation points possible with manual or automated serological methods.

Phenotype in the context of red-cell blood group and Human Platelet Antigen (HPA) refers to the expressed moiety of an allele for a given gene, and is also referred to in this document as ‘antigen’. Genotype refers to the two alleles of an autosomal gene that occupy a given locus or alternatively to either one or two alleles of an X-linked gene that occupies a given locus.

Antigen refers to a red cell or platelet membrane carbohydrate, protein or glycoprotein that is expressed as a polymorphic structure among the human population, that is to say a moiety that is immunogenic in another animal, or human, due differences in its amino acid or carbohydrate composition. Blood group or red cell, or HPA or platelet antigen refers to a moiety expressed on red cells or platelets that has been assigned a blood group or Human Platelet Antigen (HPA) designation, or provisional or workshop designation. The present invention comprises a method and for the determination of the antigen genotype and corresponding phenotype of any blood group or red cell, or HPA or platelet antigen using multiplex PCR SNP analysis. The following two tables (Table A and Table B) list most of the known human blood group and platelet antigens. Many of the antigens can be identified by their unique nucleotide sequence.

TABLE A Human Red Cell Blood Group Systems Component Associated ISBT Name (ISBT Chromosome Gene Name Name Blood Group Number) Location ISGN (ISBT) (CD Number) Antigens ABO (001) 9q34.2 ABO (ABO) Carbohydrate A, B, A, B, A1 MNS (002) 4q28.2-q31.1 GYPA (MNS) GPA (CD235a) M, N, Vw, GYPB (MNS) GPB (CD235b) S, s, U, He + 36 more P (003) 22q11.2-qter P1 (P1) Carbohydrate P1 Rh (004) 1p36.13-p34.3 RHD (RH) RhD (CD240D) D, G, Tar RHCE (RH) RhCE C, E, c, e, V, (CD240CE) Rh17 + 39 more Lutheran (005) 19q13.2 LU (LU) Lutheran glyco- Lu^(a), Lu^(b), Lu3, protein Lu4, Au^(a), Au^(b) + B-CAM (CD239) 13 more Kell (006) 7q33 KEL (KEL) Kell glycoprotein K, k, Kp^(a), Kp^(b), (CD258) Ku, Js^(a), Js^(b) + 17 more Lewis (007) 19p13.3 FUT3 (LE) Carbohydrate Le^(a), Le^(b), Le^(ab), Adsorbed form Le^(bh), ALe^(b), BLe^(b) plasma Duffy (008) 1q22-q23 DARC (FY) Fy glycoprotein Fy^(a), Fy^(b), Fy3, (CD234) Fy4, Fy5, Fy6 Kidd (009) 18q11-q12 SLC14A1 (JK) Kidd Jk^(a), Jk^(b), Jk3 glycoprotein Diego (010) 17q21-q22 SLC4A1 (DI) Band 3, AE1 Di^(a), Di^(b), Wr^(a), (CD233) Wr^(b), Wd^(a), Rb^(a) + 14 more Yt (011) 7q22 ACHE (YT) Acetyl- Yt^(a), Yt^(b) cholinesterase Xg (012) Xp22.32 XG (XG) Xg^(a) glycoprotein Xg^(a) MIC2 CD99 CD99 Scianna (013) 1p34 ERMAP (SC) ERMAP Sc1, Sc2, Sc3, Rd Dombrock (014) 12p13.2-p12.1 DO (DO) Do glycoprotein; Do^(a), Do^(b), Gy^(a), ART 4 Hy, Jo^(a) Colton (015) 7p14 AQP1 (CO) Channel-forming Co^(a), Co^(b), Co3 integral protein Landsteiner- 19p13.3 LW (LW) LW glycoprotein LW^(a), LW^(ab), LW^(b) Wiener (016) (ICAM-4) (CD242) Chido/Rodgers 6p21.3 C4B, C4A C4B, C4A CH1, CH2, Rg1 + (017) (CH/RG) 6 more Hh (018) 19q13.3 FUT1 (H) Carbohydrate H (CD173) Kx (019) Xp21.1 XK (XK) Xk glycoprotein Kx Gerbich (020) 2q14-q21 GYPC (GE) GPC Ge3, Ge4, Wb, GPD (CD236) Ls^(a), Dh^(a) Ge2, Ge3, An^(a) Cromer (021) 1q32 DAF (CROM) DAF (CD55) Cr^(a), Tc^(a), Tc^(b), Tc^(c), Dr^(a), Es^(a), IFC, WES^(a), WES^(b), UMC, GUTI Knops (022) 1q32 CR1 (KN) CR1 (CD35) Kn^(a), Kn^(b), McC^(a), Sl^(a), Yk^(a) Indian (023) 11p13 CD44 (IN) Hermes antigen In^(a), In^(b) (CD44) OK (024) 19pter-p13.2 CD147 (OK) Neurothelin, Ok^(a) basogin (CD147) RAPH (025) 11p15.5 MER2 (MER2) Not defined MER2 JMH (026) 15q22.3-q23 SEMA-L (JMH) H-Sema-L JMH (CD108) I (027) 6p24 CGNT2 (IGNT) Carbohydrate I Globoside (028) 3q25 B3GALT3(βGalN Carbohydrate P AcT1) (Gb₄, globoside) GIL (029) 9p13 AQP3 (GIL) AQP3 GIL ISGN = International Society for Gene Nomenclature

TABLE B Human Platelet Antigen Systems Gene Chromosome Associated System Name Location Component Name (CD) Antigens HPA-1 GP3A 17q21.32 Integrin β3 (CD61) Pl^(A1/2) HPA-2 GP1BA 17pter-p12 Glycoprotein Ibα (CD42b) Ko^(a/b) HPA-3 GP2B 17q21.32 Integrin α2b (CD41) Bak^(a/b) HPA-4 GP3A 17q21.32 Integrin β3 (CD61) Pen^(a/b) HPA-5 GP1A 5q23-q31 Integrin α2 (CD49b) Br^(a/b) HPA-6w GP3A 17q21.32 Integrin β3 (CD61) Ca^(a)/Tu^(a) HPA-7w GP3A 17q21.32 Integrin β3 (CD61) Mo^(a) HPA-8w GP3A 17q21.32 Integrin β3 (CD61) Sr^(a) HPA-9w GP2B 17q21.32 Integrin α2b (CD41) Max^(a) HPA- GP3A 17q21.32 Integrin β3 (CD61) La^(a) 10w HPA- GP3A 17q21.32 Integrin β3 (CD61) Gro^(a) 11w HPA- GP1BB 22q11.2 Glycoprotein Ibβ (CD42c) Ly^(a) 12w HPA- GP1A 5q23-q31 Integrin α2 (CD49b) Sit^(a) 13w HPA- GP3A 17q21.32 Integrin β3 (CD61) Oe^(a) 14w HPA-15 AF410459 6q13 GPI-linked GP (CD109) Gov^(a/b) HPA- GP3A 17q21.32 Integrin β3 (CD61) Duv^(a) 16w ? GPV ? Glycoprotein V Pl^(T) ? GPIV 7q11.2 Glycoprotein IV (CD36) Vis^(a)/Nak^(a) Note: HPA numbers on the left ending with a ‘w’ represent ISBT workshop designations and are tentative HPA systems.

A single nucleotide polymorphism (SNP) refers to any blood group or HPA allele that defines a specific red cell or platelet antigen by virtue of its unique nucleotide sequence as defined in Garratty et al. Transfusion 2000; 40:477 and as updated from time-to-time by the International Society of Blood Transfusion.

It is understood that the presently disclosed subject matter is not limited to the particular methodology, protocols, cell lines, vectors, and reagents described as these can vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the presently disclosed subject matter.

Unless defined otherwise, all technical and scientific terms used herein are intended to have their meanings as understood by one skilled in the present art. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the presently disclosed subject matter, the preferred embodiments, methods, devices and materials described.

It is also understood that the articles ‘a’ and ‘an’ are used herein to refer to one or to more than one (i.e. to at least one) of the grammatical object of the article. Accordingly, ‘an element’ means one element or more than one element.

Our novel platform simultaneously performs automated multiple blood group-associated SNP analyses using genomic DNA and the Thermus aquaticus polymerase chain reaction (PCR) to infer the presence of specific blood group genotypes. This automated high-throughput platform has particular application in the blood donation industry since it represents a novel screening tool for the expression of blood group antigens or phenotypes.

Our platform provides important genotypic information within 24 hours of donation. When performed on all blood donations for all important blood group phenotypes, our invention will provide a comprehensive database to select and confirm blood group phenotypes using government regulated antisera. The use of this platform as a screening tool will lessen the number of regulated blood group phenotype tests done by the collection facility and end user, and meet the end user demand for antigen-matched blood for transfusion recipients.

Unique to this invention is the assay design for the simultaneous identification of a plurality of blood group or HPA alleles. The present invention provides novel assay for the simultaneous identification of a plurality of blood group or HPA alleles, and more preferably of 19 blood group alleles using a plurality of SNPs, and more preferably, 12 SNPs. In one embodiment, the genotyping platform queries genetic variants using multiplexed single nucleotide primer extension coupled with two-laser fluorescence detection and software for automated genotype calling. Each of the relevant gene regions are PCR amplified from purified genomic DNA in a single reaction using the following oligonucleotide primer designs:

Gene Primer Sequence (5′ - 3′) RHD Exon 4 RHDe4S AGACAAACTGGGTATCGTTGC RHDe4A ATCTACGTGTTCGCAGCCT RHD Exon 9 RHDe9S CCAAACCTTTTAACATTAAATTATGC RHDeDA TTGGTCATCAAAATATTTAGCCTC RHCE Exon 2 RHCEe2S TGTGCAGTGGGCAATCCT RHCEe2A CCACCATCCCAATACCTG RHCE Exon 5 RHCEe5S AACCACCCTCTCTGGCCC RHCEe5A ATAGTAGGTGTTGAACATGGCAT GYPB Exon 4 GYPBe4S ACATCTCTTTCTTATTTGGACTTAC GYPBe4A TTTGTCAAATATTAACATACCTGGTAC KEL Exon 6 KELe6S TCTCTCTCCTTTAAAGCTTGGA KELe6A AGAGGCAGGATGAGGTCC KEL Exon 8 KELe8S AGCAAGGTGCAAGAACACT KELe8A AGAGCTTGCCCTGTGCCC FY Promoter FYproS TGTCCCTGCCCAGAACCT FYproA AGACAGAAGGGCTGGGAC FY Exon 2 FYe2S AGTGCAGAGTCATCCAGCA FYe2A TTCGAAGATGTATGGAATTCTTC JK Exon 9 JKe9S CATGAACATTCCTCCCATTG JKe9A TTTAGTCCTGAGTTCTGACCCC DI Exon 18 DIe19S ATCCAGATCATCTGCCTGG DIe19A CGGCACAGTGAGGATGAG GP3A GP3Ae3S ATTCTGGGGCACAGTTATCC GP3Ae3A ATAGTTCTGATTGCTGGACTTCTC

The above primer pairs comprise the corresponding forward and reverse primers, and may be referred to herein as SEQ ID NOs 1-24.

Multiplexed single nucleotide primer extension is performed using the following 5′ tagged extension primers:

RHD Exon 4 GTGATTCTGTACGTGTCGCCGTCTGATCTTTATCCTCCGTTCCCT RHD Exon 9 GCGGTAGGTTCCCGACATATTTTAAACAGGTTTGCTCCTAAATCT RHCE Exon 2 GGATGGCGTTCCGTCCTATTGGACGGCTTCCTGAGCCAGTTCCCT RHCE Exon 5 CGACTGTAGGTGCGTAACTCGATGTTCTGGCCAAGTGTCAACTCT GYPB Exon 4 AGGGTCTCTACGCTGACGAT TTGAAATTTTGCTTTATAGGAGAAA KEL Exon 6 AGCGATCTGCGAGACCGTATTGGACTTCCTTAAACTTTAACCGAA KEL Exon 8 AGATAGAGTCGATGCCAGCTTTCCTTGTCAATCTCCATCACTTCA FY Promoter GACCTGGGTGTCGATACCTAGGCCCTCATTAGTCCTTGGCTCTTA FY Exon 2 ACGCACGTCCACGGTGATTTGGGGGCAGCTGCTTCCAGGTTGGCA JK Exon 9 CGTGCCGCTCGTGATAGAATAAACCCCAGAGTCCAAAGTAGATGT DI Exon 19 GGCTATGATTCGCAATGCTTGTGCTGTGGGTGGTGAAGTCCACGC GP3A Exon 3 AGAGCGAGTGACGCATACTTGGGCTCCTGTCTTACAXGCCCTGCCTC

The above probes may be referred to herein as SEQ ID NOs 25-36. The DNA bases are represented by their single letter equivalents (A, C, G or T) and the letter X represents a C3 (phosphoramidite) spacer between the two adjacent DNA bases.

In this embodiment, the 12 bolded nucleotides in the 5′ region of the extension probes are hybridized to a complementary DNA sequence that has been micro-arrayed onto microplates so that specific blood group SNPs are individually identified and reported.

Proof of principle experiments have been performed using 372 consent qualified samples (please refer to Appendix A). Collection of serological data for samples has been constant and the success rates based upon the expected allele frequencies have been performed.

In the preceding example, one preferred embodiment has been described. However, it should be obvious to one skilled in the art that other methodologies and/or technologies for SNP identification could be used, providing that the novel DNA sequences disclosed above are also used.

The teachings and method of the present invention are superior to the teachings of the prior art for a number of reasons, one of which is that the complete method of the present invention, from DNA extraction to result computation analyses can be automated and multiplexed so that many SNPs can be determined simultaneously. This automated multiplex high throughput analysis can meet the demand (hundreds of blood donations can be tested) and the turn-around time (<24 hours) to collate and provide valuable information to the blood collection facility before blood is shipped to the end user. This platform and method has the further advantage over existing technology in that it reduces operator handling error.

In addition, there are significant cost reductions compared with the current technology. The invention addresses the need for an automated, accurate, rapid and cost-effective approach to the identification of multiple blood group SNPs. According to an embodiment, a multiplex SNP assay of the present invention detected 12 SNPs overnight on 372 individual blood samples. In accordance with the teachings of the present invention, the platform, products and methods of the present invention can detect all SNP variations for all blood group antigens, for example, as shown below on 744 samples.

BRIEF DESCRIPTION OF THE DRAWINGS

Further features and advantages of the present invention will become apparent from the following detailed description, taken in combination with the appended drawings, in which:

FIG. 1 A computer screen display of a typical UHT SNP scatter plot to sort the fluorescence of a C/T SNP analysis of GP3A Exon 3 for HPA-1a/b genotyping.

FIG. 2 Representative samples of GP3A Exon 3 HPA-1a/b) genotyping by manual PCR-RFLP analysis using MspI restriction enzyme analysis (A) and the tabulated comparative results with the UHT SNP analysis (B).

FIG. 3 Representative samples JK genotyped by manual PCR-RFLP analysis using MnlI (A) and the tabulated comparative results with the UHT SNP analysis (B).

FIG. 4 A-L Computer screen displays of typical UHT SNP scatter plots to sort the fluorescence of a C/T SNP for various blood group and HPA genotypes.

Appendix A provides a tabulated summary of the multiplex SNP assay detection of 12 possible SNPs on 372 individual blood samples.

It will be noted that throughout the appended drawings, like features are identified by like reference numerals.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). The present invention provides a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform, or any other SNP analysis system, to genotype blood for a plurality of common antigen-associated SNPs, including but not limited to: RhD (2), RhC/c, RhE/e, S/s, K/k, Kp^(a/b), Fya/b, FY0, Jk^(a/b), Di^(a/b), and HPA-1a/b. According to one example of the present invention, a total of 372 samples were analysed for 12 SNPs overnight. Individual SNP pass rates varied from 98-100% for 11 of 12 SNPs. Of the Rh-pos, 98.6% were correctly identified. Six of 66 Rh-neg (9%) were typed as RHD-pos; 5 of 6 were subsequently demonstrated to contain an non-RHDψ gene by SSP-PCR. Eleven of 12 R1R1 and 1 of 1 r″r were correctly identified. HPA-1b was identified in 4, which was confirmed by PCR-RFLP (n=4) and serology (n=1). PCR-RFLP on selected samples (n<20) for K/k, Fy^(a/b), and Jk^(a/b) were 100% concordant. Confirmation of some of the results is provided. The platform has the capacity to genotype thousands of samples per day for all SNP variations. The suite of SNPs can provide collection facilities with real-time genotypic data for all donors at an annual cost (excluding RhD) estimated to equal the current cost of phenotyping 5-10% of the donors.

Methods and Reagents Methodology Specific to the Invention.

We have designed a novel blood group and HPA SNP and detection system that employ the use of two sets of novel compounds (reagents) that are specifically designed to work in a multiplex format.

In brief, genomic DNA is harvested the salting out procedure using the Qiagen (Qiagen Inc. Valencia, Calif.) Blood DNA Isolation Kit. Our invention can use any good quality DNA harvested by any one of a variety of methods. For the multiplex PCR, the DNA regions containing all 12 SNPs of interest were PCR-amplified in a single reaction well. Tables 1 and 2 outline the novel PCR primers and extension probes, respectively, used in the assay. Note that the concentration of the various reagents may be adjusted to optimize DNA amplification, and is dependent on but is not limited to: the concentration and quality of the genomic DNA, the concentration of the PCR primers or the type of thermal cycler used for the PCR.

Our current genotyping technology identifies SNPs using single base-pair primer extension using the novel products and protocols of the present invention. In brief, the genomic region surrounding the SNP of interest is PCR-amplified as described above, preferably using one or more, or all of the primer pairs of Table 1. Then, the amplified DNA fragments are used as a template for DNA hybridization using one or more or all the corresponding novel probes of Table 2, and single nucleotide extension (synthesis) based on the nucleotide present at each of the specific SNP sites. The PCR primers pairs in Table 1 represent sequences complementary to DNA regions containing SNPs of interest; of which the exact sequences of each primer pair and mixture of primer pairs have been specifically optimized to amplify genomic DNA of interest as a mixture of 12 primer pairs. Although noted above, Table 2 further summarizes 12 novel extension primers specifically used together to detect the nucleotides of blood group and platelet antigen or HPA SNPs, simultaneously. The extension primers represent a group of 12 novel nucleotide sequences, of which each are a combination of: 1) a unique 5′ region necessary to direct hybridization to a micro-arrayed tag located in a specific spot in each microplate well, and 2) a 3′ region complementary to and adjacent to a SNP of a PCR-amplified DNA region containing the SNP of interest.

TABLE 1 The PCR primers used in the 12-pair multiplex PCR format for multiple SNP detection. Primer Product Size Antigen SNP Name Sequence 5′-3′ Target (bp) RhD/RhCE C/T RHDe4S AGACAAACTGGGTATCGTTGC RHD 111 RHDe4A ATCTACGTGTTCGCAGCCT Exon 4 RhD/RhCE A/G RHDe9S CCAAACCTTTTAACATTAAATTATGC RHD 98 RHDe9A TTGGTCATCAAAATATTTAGCCTC Exon 9 RhC/Rhc T/C RHCEe2S TGTGCAGTGGGCAATCCT RHCE 90 RHCEe2A CCACCATCCCAATACCTG Exon 2 RhE/Rhe C/G RHCEe5S AACCACCCTCTCTGGCCC RHCE 107 RHCEe5A ATAGTAGGTGTTGAACATGGCAT Exon 5 GYPBS/GYPBs T/C GYPBe3S ACATGTCTTTCTTATTTGGACTTAC GPYB 103 GYPBe3A TTTGTCAAATATTAACATACCTGGTAC Exon 4 K/k T/C KELe6S TCTCTCTCCTTTAAAGCTTGGA KEL 142 KELe6A AGAGGCAGGATGAGGTCC Exon 6 Kp^(a)/Kp^(b) T/C KELe8S AGCAAGGTGCAAGAACACT KEL 100 KELe8A AGAGCTTGCCCTGTGCCC Exon 8 Fy/Fy0 T/C FYproS TGTCCCTGCCCAGAACCT Duffy 90 FYproA AGACAGAAGGGCTGGGAC Promoter Fy^(a)/Fy^(b) G/A FYe2S AGTGCAGAGTCATCCAGCA Duffy 122 FYe2A TTCGAAGATGTATGGAATTCTTC Exon 2 Jk^(a)/Jk^(b) G/A JKe9S CATGAACATTCCTCCCATTG Kidd 130 JKe9A TTTAGTCCTGAGTTCTGACCCC Exon 9 Di^(a)/Di^(b) T/C DIe19S ATCCAGATCATCTGCCTGG Diego 90 Die19A CGGCACAGTGAGGATGAG Exon 19 HPA-1a/b T/C GP3Ae3S ATTCTGGGGCACAGTTATCC GP3A 114 GP3Ae3A ATAGTTCTGATTGCTGGACTTCTC Exon 3 The above pimers may be referred to herein as SEQ ID NOs 1-24.

TABLE 1A Additional Blood Group and Platelet Antigen SNPs for Clinically Relevant Antigens. Product Size Antigen SNP Target (bp) A/O G/T ABO GalNAc/Del Exon 6 A/B C/G ABO (GalNAc/Gal) Exon 7 A/B G/A ABO (GalNAc/Gal) Exon 7 A/B C/A ABO (GalNAc/Gal) Exon 7 A/B G/C ABO (GalNAc/Gal) Exon 7 M/N G/A MNS Exon 2 M/N T/G MNS Exon 2 MNS/MiI C/T MNS Exon 3 RHD/Weak D T/G RHD Type 1 Exon 6 RHD/Weak D G/C RHD Type 2 Exon 9 RHD/Weak D C/G RHD Type 3 Exon 1 RHD/D nt602 C/G RHD Variants Exon 4 RHD/‘DAR’ T/C RHD Variant Exon 7 RHD/Weak D C/A RHD Type 5 Exon 3 RHD/D_(el) G/A RHD IVS3 + 1 RHD/D_(el) G/T RHD Exon 6 RHD/D_(el) G/A RHD Exon 9 RHD/RHDψ A/T RHD nt506 Exon 4 RHCE/RhC T/C RHCE IVS2 + 1722 RHCE/RhC C/T RHCE IVS2_1751 RHCE/ C/G RHCE VS variant Exon 5 Lu^(a)/Lu^(b) A/G LU Exon 3 Au^(a)/Au^(b) A/G LU Exon 12 Js^(a)/Js^(b) C/T KEL Exon 17 Js/Js_(null) G/T JK IVS7 + 1 FY/Fy^(x) C/T FY Exon 2 FY/Fy^(x) G/A FY Exon 2 Wr^(a)/Wr^(b) A/G DI Exon 16 Yt^(a)/Yt^(b) C/A YT Exon 2 Sc1/Sc2 G/A SC Exon 3 Do^(a)/Do^(b) C/T DO (nt 378) Exon 2 Do^(a)/Do^(b) T/C DO (nt 624) Exon 2 Do^(a)/Do^(b) A/G DO (nt 793) Exon 2 Co^(a)/Co^(b) C/T CO Exon 1 In^(a)/In^(b) C/G IN Exon 2 Ok(a+)/Ok(a−) G/A OK Exon 4 GIL/GIL_(null) G/A GIL IVS5 HPA-2a/b C/T GP1BA Exon 2 HPA-3a/b T/G GP2B Exon 26 HPA-4a/b G/A GP3A Exon 4 HPA-5a/b G/A GP1A Exon 13 Gov^(a)/Gov^(b) A/C CD109 Exon 19

Each antigen listed on the left represents a blood group or HPA genotype and the single nucleotide polymorphism (SNP). Some genotypes are evaluated using more than one SNP because they differ by more than one nucleotide. Each PCR primer pair consists of a sense (Primer Name ending in S) and antisense (Primer Name ending in A) oligonucleotide (Sequence 5′-3′) designed to amplify the DNA region containing the SNP for the antigen of interest. The target region (Product Target) and the amplified fragment (Size (bp)) are shown on the right. Note that 12 SNPs are evaluated for 19 different blood group and platelet antigens because some antigens have more than one SNP. In some cases an A or G SNP is included since the complementary DNA strand can be evaluated as it will contain the T or C SNP of interest.

TABLE 2 Extension probes used to detect the nucleotides of blood group and HPA SNPs. Name Sequence 5′-3′ RHD Exon 4 GTGATTCTGTACGTGTCGCC GTCTGATCTTTATCCTCCGTTCCCT RHD Exon 9 GCGGTAGGTTCCCGACATAT TTTAAACAGGTTTGCTCCTAAATCT RHCE Exon 2 GGATGGCGTTCCGTCCTATT GGACGGCTTCCTGAGCCAGTTCCCT RHCE Exon 5 CGACTGTAGGTGCGTAACTC GATGTTCTGGCCAAGTGTCAACTCT GYPB Exon 4 AGGGTCTCTACGCTGACGAT TTGAAATTTTGCTTTATAGGAGAAA KEL Exon 6 AGCGATCTGCGAGACCGTAT TGGACTTCCTTAAACTTTAACCGAA KEL Exon 8 AGATAGAGTCGATGCCAGCT TTCCTTGTCAATCTCCATCACTTCA FY Promoter GACCTGGGTGTCGATACCTA GGCCCTCATTAGTCCTTGGCTCTTA FY Exon 2 ACGCACGTCCACGGTGATTT GGGGGCAGCTGCTTCCAGGTTGGCA JK Exon 9 CGTGCCGCTCGTGATAGAAT AAACCCCAGAGTCCAAAGTAGATGT Di Exon 19 GGCTATGATTCGCAATGCTT GTGCTGTGGGTGGTGAAGTCCACGC GP3A Exon 3 AGAGCGAGTGACGCATAC TTGGGCTCCTGTCTTACAXGCCCTGCCTC The above probes may be referred to herein as SEQ ID NOs 25-36. The DNA bases are represented by their single letter equivalents (A, C, G or T) and the letter X represents a C3 (phosphoramidite) spacer between the two adjacent DNA bases.

The present invention also provides novel hybrid probes, wherein the preferred probes are listed in Table 2, but limited to said listing. Each extension probe is designed in two parts: (1) the 5′ portion: the 5′ nucleotides indicated in boldface of the extension primer are complementary to unique and specific DNA sequences which are micro-arrayed onto the bottom of microplates in a specified location of each microplate well. Thus, the 5′ portion of the extension probes in table 2 represent, but are not limited to, 12 unique complementary sequences used together to identify the individual SNPs through hybridization to the micro-arrayed tags in the microplate wells. The 12 unique 5′ portions can be interchanged with each of the 3′ regions specified below, which contain DNA sequences complementary to and adjacent to the SNPs of interest, or they can be interchanged with other additional unique 5′ portions as specified by the micro-arrayed tags in the microplate wells provided they are used to identify blood group or HPA SNPs; and (2) the 3′ portion: the 3′ nucleotides are complementary to and precisely adjacent to the SNP site of the PCR-amplified DNA, which enables the detection of either or both nucleotides of the SNP. Thus, the extension probe is a unique sequence that can hybridized to a specific location and to the PCR-amplified DNA and be extended by a single fluorescent-labeled dideoxy-nucleotide using PCR thermal cyclers. The extension probe products are hybridized to the complementary micro-arrayed DNA sequence on the microplate and the incorporation of Bodipy- and Tamra-labeled dideoxy-nucleotides are detected by laser-microplate fluorescence for each individual blood group SNP. The presence of the nucleotides for a given SNP is displayed by automated imaging and analysis software. In one variation of the detection reaction, a dideoxyguanidine tri-nucleotide labeled with the Bodipy-fluorochrome is added in the extension reaction. If a deoxycytidine is present in the PCR-amplified DNA fragment, then the nucleotide will be incorporated into the nascent DNA fragment. In another variation of the reaction, a dideoxyadenine nucleotide labeled with the Tamra-fluorochrome is added to the extension assay. If the PCR-amplified fragment contains a deoxythimidine, then an extension will occur. In each case, the flurochrome is detected after the extension reaction has been completed. Again, these reactions proceed in the same tube along with the other extension reactions. The laser-detection apparatus can identify and evaluate each specified extension due to the location of each micro-arrayed DNA sequence.

Each extension primer has a region complementary to a tag that is been bound to the surface of a microplate well (Bold nucleotides) and a region (Italicized nucleotides) that is complementary to the region and immediately adjacent to the SNP site.

It should be noted that the teachings, products and methods of the present invention are not limited to the above-specified primer pairs and probes, but additionally comprise all primer pairs and probes specific to the blood group and HPA SNPs, wherein said primer pairs and probes are optimized for use in a multiplex PCR reaction for the simultaneous identification of more than one, or all, blood group or HPA genotypes and their corresponding phenotypes.

EXAMPLES

Although the following examples may provide preferred methods, products, platforms or protocols of the present invention, it will be understood by one skilled in the art that the presently provided examples are not limited to the specified parameters of each example, and may be varied provided that the resulting outcome of the methods or protocols are in accordance with the teachings of the present invention, and the products are functionally equivalent or relating to the teachings of the present invention.

Example 1

A preferred protocol for the multiplex blood group and HPA SNP Genotyping is provided. Although the present example analyzes 12 SNP extension primers, the present invention is not limited to the analysis of a maximum of 12 SNPs, but may include a plurality of SNPs relating to more than one or all of the blood group or HPA SNPs.

Additional blood group and platelet antigen SNPs for clinically relevant antigens embodied by the present invention appear in Table 1A. Primer pairs and probes, such as those exemplified in Tables 1 and 2, corresponding to these SNPs of clinical relevance, can be prepared according to the teachings of the present invention. Target primers may be initially identified from existing databases (e.g. autoprimer.com) based on information corresponding to the SNP of interest and the corresponding flanking regions, and subsequently optimized as herein disclosed for use in accordance with the present invention.

I (a). PCR Primer Pooling Step Action 1 Dilute each of 12 PCRS and PCRA primer (forward and reverse primers) pairs to final concentration of 240 uM (only required upon arrival of new primers) 2 Generate working primer pool by combining 5 ul of each of the 24 individual PCR primers

I (b). SNP Extension Primer Pooling Step Action 1 Dilute each of 12 SNP extension primers to final concentration of 120 uM (only required upon arrival of new primers) 2 Generate working SNP extension primer pool by combining 10 ul of each of the 12 individual SNP extension primers

II. Multiplex PCR from purified DNA templates Step Action 1 Prepare 10 ul multiplex PCR master mix for use with 96 well plates containing PCR primers (synthesized by Integrated DNA Technologies, Coralville, IA, USA), dNTPs (MBI Fermentas, Hanover, MD, USA), MgCl₂, 10X PCR Buffer, and Amplitaq Gold (Applied Biosystems, Branchburg, NJ, USA): Initial Final Volume Component Concentration Concentration (ul/well) PCR primer pool 0.05 10 uM each 50 nM each dNTPs 2.5 mM each 75 uM each 0.33 MgCl₂ 25 mM 5 mM 2.00 10x PCR Buffer 10x 1x 1.00 AmpliTaq Gold 5U/ul 0.075U/ul 0.15 dH₂O 4.47 2 For each DNA Sample, transfer 2 ul of 4 ng/ul stock DNA to each well of 96 well plates. Use Biomek FX (Beckman Coulter Inc., Fullerton, CA, USA) Script ‘2ul96well Transfer’ automated program 3 Place Multiplex PCR Master Mix in Biomek FX station 1. Place 96 well plates of DNA in Biomek FX station 5-8. 4 Transfer 8 ul Multiplex PCR master mix to DNA samples using Biomek FX Script: ‘8ul PCR Transfer’ 5 After addition of master mix seal tightly with MJ Microseal A film (MJ Research, Inc., Waltham, MA, USA) 6 Spin down in centrifuge for 30 sec at 1500 rpm 7 Place in MJ Tetrad Thermal cyclers (MJ Research, Inc., Waltham, MA, USA) and run ‘UHT-MPX’ CBS multiplex PCR program: Thermal cycle conditions ‘UHT-MPX’: Denature 94° C. 1:00 (min) 35 cycles of: 94° C. 0:30 (min) 55° C. 0:33 (min) 72° C. 1:00 (min) Hold Temperature  4° C. ∞

III. Post PCR Cleanup Step Action 1 Prepare ExonucleaseI (ExoI; USB Corporation, Cleveland, OH, USA) and Shrimp Alkaline Phosphatase (SAP; USB Corporation, Cleveland, OH, USA) master mix: Component Final concentration Volume per well (ul) ExoI 2U 0.4 SAP 1U 2.0 10x SAP buffer 1x 0.6 dH₂O 3.0 2 Add Exo/SAP master mix to grooved reservoir and place on Multimek (Beckman Coulter Inc., Fullerton, CA, USA) Station 3 3 Add UHT (ultra high-throughput) salt solution (provided) to grooved reservoir and place on Multimek Station 4 4 Transfer 8 ul Exo/SAP master mix to amplified PCR products using Multimek Script: EXO96-2.SCI (two 96 well plates, at Multimek stations 1 and 2 5 After Multimek addition of Exo/SAP seal tightly with MJ Microseal A film 6 Spin down in centrifuge for 30 sec at 1500 rpm 7 Place in MJ Tetrad Thermal cyclers and run ‘UHTCLEAN’ program: Thermal cycle conditions ‘UHTCLEAN’: Temp Time (min) 37° C. 30:00 100° C. 10:00  4° C. ∞

IV. SNP-IT Assay using the GenomeLab SNPStream ™ (Beckman Coulter Inc. Fullerton, CA, USA) Step Action 1 Prepare SNP-IT extension mix containing extension primers (synthesized by Integrated DNA Technologies, Coralville, IA, USA), C/T ddNTPs, Extension mix diluent, and DNA polymerase (Beckman Coulter Inc., Fullerton, CA, USA) Component Volume per well (ul) SNP Extension primer pool  3.22 C/T ddNTP Extension mix  21.43 Extension mix diluent 402.98 DNA polymerase  2.24 dH2O 318.22 2 Add SNP-IT mix to grooved reservoir and place on Multimek Station 3 3 Add UHT salt solution (provided) to grooved reservoir and place on Multimek Station 4 4 Transfer 7 ul SNP-IT extension mix to UHT-CLEAN PCR products using Multimek Script: 7UL96-2.SCI (two 96 well plates, at Multimek stations 1 and 2 5 After Multimek addition of SNP-IT extension mix seal tightly with MJ Microseal A film 6 Spin down in centrifuge for 30 sec at 1500 rpm 7 Place in MJ Tetrad Thermal cyclers and run ‘UHT- SNPIT’ program: Thermal cycle conditions ‘UHTSNPIT’: Temp Time (min) Denature 96° C. 3:00 45 cycles of: 94° C. 0:20 40° C. 0:11 Hold Temperature  4° C. ∞

V. Post-extension Transfer and Hybridization Step Action 1 Preheat incubator to 42° C. 2 Make sure there is adequate 20x dilution of SNPWare UHT Wash Buffer in washer Carboy B. If required dilute 20x stock solution with water and refill Carboy B 3 Run SAMI/EL 405 Script ‘Prime B’ 4 Place all Tag Array plates in Row 1 of the Carousel, starting with Hotel 1, with subsequent plates in Hotel 2, 3, etc., preferably with their barcodes facing inwards. 5 Place all PCR plates directly below their corresponding Tag Array Plates. PCR plates corresponding to Quadrants 1-4 should be placed in Rows 2-5 of the proper Hotel, respectively. For all PCR plates, the “ABC . . . ” lettered edge of the plates should face inwards on the Carousel. 6 Place grooved reservoir with solubilized UHT Salt Solution in Multimek Station 4 7 Place grooved reservoir with Hybridization solution master mix in Multimek Station 3 Hybridization Solution master mix: Component (ul) Volume per Tag Array plate 2x Hybridiaztion Soluton 3500.00 Hybridization Additive  203.7 8 Run SAMI Script ‘Post-extension Transfer_Hybridization 1x384.smt’: This automated program prepares the tag array plate by washing it 3x with SNPWare UHT wash buffer; adds 8.0 ul of Hybridization solution master mix to each SNP extension reaction and subsequently transfers 8.0 ul of this mixture to the prepared tag array plate. 9 Place Tag Array plates in humidified 42° C. incubator for 2 hours

VI. Post-Hybridization Wash Step Action 1 Make sure there is adequate 64x dilution of SNPWare UHT Stringent Wash Solution in washer Carboy C. If required dilute 64x stock solution with water and refill Carboy C 2 Run SAMI/EL 405 Script ‘Prime C’ 3 Run SAMI/EL 405 Script ‘Post-hyb 3x Wash’ 4 Completely dry Tag Array plates using vacuum/pipette tip 5 Run SAMI/EL405 script ‘Prime A’ several times to clean plate washer pins

VII. UHT (Ultra high through-put) Tag Array Plate Reading Step Action 1 Turn on lasers, turning both keys 90 degrees clockwise, and allow at least 30 minutes to warm up 2 Turn on SNPScope Reader and Twister. 3 Activate lasers: Flip two switches on laser box from ‘Standby’ to ‘Operate’/‘Laser’ 4 Open UHT Run Manager Software and ‘Initialize’ SNPScope system 5 Stack Tag Array plates in Twister carousel 1, with ‘Assay Test Plate’ on top. Make sure all barcodes are facing outwards, and plates are pushed towards the reader 6 Select ‘SNPTEST_W_BC_run’ from UHT RUN Manager Software, enter the number of plates to be read (including the test plate). 7 Select ‘RUN’

The SNPScope plate reader will excite and capture images of Bodipy-fluorescein and Tamra-labeled ddNTPs separately. All genotype calls are subsequently automatically generated using the SNPStream Software Suite of MegaImage, UHTGetGenos and QCReview.

It should be noted that the specific steps associated with the protocol exemplified in Example 1 are not intended to limit the teachings and methods of the present invention to the specific above protocol. Example 1 is provided to specify a preferred method in accordance with the present invention wherein a plurality of blood group and HPA SNPs are simultaneously analysed in a ultra high throughput multiplex automated system for the determination of the specific genotypes and accordingly the phenotypes associated therewith. Accordingly, it should be understood by one skilled in the art that the steps of Example 1 may be varied provided that such variations yield the preferred results of the present invention.

Results 1. GP3A Exon 3 SNP Scatter Plots.

The robotic UHT platform produces laser-fluorescence values for each sample which are represented in ‘scatter plots’ for the operator to review. A sample scatter plot is shown in FIG. 1 for the SNP analysis GP3A Exon 3, which represents the HPA-1a and HPA-1b antigens. As can been seen in FIG. 1 and FIG. 4, results are graphed using logarithmic and XY scatter plots (upper right). Green, orange or blue sample designations represent CC, TC and TT SNP genotype calls, respectively. No fluorescence represents an assay failure (FL) for that sample.

Scatter plots (as shown in FIG. 1 and FIG. 4) are generated preferably using SNPStream software suite and viewed through QCReview. It should be additionally noted that the present analysis is not limited to SNPstream or QCReview, and may be carried out using any SNP analysis software. Individual TT, TC and CC genotype calls are represented as dark blue, orange and green open circles, respectively. Sample failures and water controls are represented by yellow and light blue filled circles respectively. Logarithmic (left) and XY scatter (upper right) plots are generated using the relative fluorescence of the Bodipy-fluorescein and Tamra labels obtained during SNPScope plate imaging and analysis.

2. SNP Data Manipulation and Analysis.

The SNP results of a scatter plot are electronically exported to a spreadsheet and examined for total sample failure and individual SNP failure rates. SNP results for 372 DNA samples are summarized in Table 3 (provided in Appendix A) Accordingly, Table 3 provides the Pass and Failure Rates for 12 blood group and HPA SNP analyses. 372 DNA samples were analyzed for several antigens, including the blood group RhD (RHD Exon 4 and RHD Exon 9) and platelet HPA-1a/b (GP3A Exon 3) genotypes. Sample success or pass rates are indicated on the right and individual SNP success or pass rates are shown at the bottom. Three hundred and fifty seven of 372 samples (96%) had results for at least one SNP. Individual SNP results (i.e. minus the sample failures) ranged from 80-100%; only two SNPs had success rates <98%. Individual SNP failures do not affect the results of a sample for other SNPs that do not fail.

3. SNP Allele Result Compared to the Serological Result

RhD status was compared between the serological result and the SNP analysis for RHD Exon 4 and RHD Exon 9. Table 4 summarizes the comparison. 287 of 291 (98.6%) RhD positive units and 55 of 66 (83.3%) RhD negative units were identified correctly using the UHT SNP platform. It is important to note that the 6 incorrect calls suggesting the presence of the RHD gene in a serologically RhD-negative sample may be due to one of the non-functional RHD genes present in the random population (Singleton B. K. et al., Blood 2000; 95:12; Okuda H., et al., J Clin Invest 1997; 100:373; Wagner F. F. et al., BMC Genet 2001; 2:10).

TABLE 4 A comparison of the SNP genotype result and the serological result obtained with government-regulated antisera. D- RHD Exon RHD Exon positive: Assay 4 9 No Percent N = 291 pos Pos 287 98.6% neg Neg 4 1.4% Total 291 D- negative: Assay RHD4 RHD9 No Percent N = 66 neg Neg 55 83.3% neg FL 5 7.6% pos Pos 6 9.1% Total 66 NOTE: CBS laboratory regulations do not allow copies of serological results of blood donors to be made from their laboratory information system. Therefore, the results of the CBS serological phenotypes were reviewed by research personnel and the results tabulated and compared to the SNP data.

4. SNP Genotype Frequency Analysis.

The SNP results then were compared with published phenotype frequencies for Caucasians and Blacks and are summarized in Table 5 below. The data clearly shows that the allele frequencies are consistent with the accepted published frequencies for Caucasians and Blacks. The data show that the SNP genotype frequencies match the published population phenotype frequencies.

Table 5. A summary of the UHT SNP analysis of genotype frequencies for several SNPs analyzed and compared to published phenotype frequencies for Caucasians and Blacks. The ethnicity of the samples analyzed is not known.

TABLE 5 UHT Genotyping Analysis FL = assay failure Phenotype Caucasians Blacks Observed (%) KEL Exon6 K− k+  91%   98% 326 91.3 K+ k−  0.2% rare 0 0 K+ k+  8.8%   2% 28 7.8 Fails 3 0.8 No of FL  18 No. of Pass 354 Call Rate 95.2% An independent assay as described in Molecular Protocols in Trans- fusion Medicine was performed using the UHT SNP Stream System. Seven samples were tested (Four KEL 2/KEL 2, Three KEL1/KEL 2). All samples showed a 100% correspondence with the UHT genotype results. KEL Exon8 Kp(a+ b−) Rare   0% 0 0 Kp(a− b+) 97.7%  100% 354 99.2 Kp(a+ b+)  2.3% rare 1 0.3 Fails 2 0.6 No of FL  17 No. of Pass 355 Call Rate 95.4% DI Exon18 Di(a+ b−) <0.01%  <0.01%  0 0 Di(a− b+) >99.9%  >99.9%  353 98.9 Di(a+ b+) <0.1% <0.1% 2 0.6 Fails 2 0.6 No of FL  17 No. of Pass 355 Call Rate 95.4% FY PRM wt/wt 348 97.5 wt/mut 7 20 mut/mut 2 0.5 Fails 0 0 No of FL 15 No. of Pass 357 Call Rate 96.0% An independent assay as described in Molecular Protocols in Trans- fusion Medicine was performed using the UHT SNP Stream System. Thirteen samples were tested (six wt/wt, five wt/mut and two mut/mut for the GATA site). All samples showed a 100% correspondence with the UHT genotype results. FY Exon 2 Fy(a+ b−)   17%   9% 89 24.9 Fy(a− b+)   34%   22% 112 31.4 Fy(a+ b+)   49%   1% 155 43.4 Fails 1 0.3 No of FL  16 No. of Pass 356 Call Rate 95.7% An independent assay as described in Molecular Protocols in Trans- fusion Medicine was performed using the UHT SNP Stream System Eleven samples were tested (eight FY2/FY2, three FY1/FY2 and one FY1/FY1). All samples showed a 100% correspondence with the UHT genotype results. GP3A Exon 3 HPA-1a/1a   80%   84% 263 73.7 HPA-1a/1b   18%   64% 89 24.9 HPA-1b/1b   2%   0% 4 1.1 Fails 1 0.3 No of FL  16 No. of Pass 356 Call Rate 95.7% An independent assay as described in Molecular Protocols in Trans- fusion Medicine was performed using the UHT SNP Stream System. Eighteen samples were tested (Seven HPA-1a, Seven HPA-1a/1b and Four HPA-1b). All samples showed a 100% correspondence with the UHT genotype results. JK9 Jk(a+ b−) 26.3% 51.1% 90 25.2 Jk(a− b+) 23.4%  8.1% 87 24.4 Jk(a+ b+) 50.3% 40.8% 178 49.4 Fails 2 0.5 No of FL  17 No. of Pass 355 Call Rate 95.4% An independent assay as described in Molecular Protocols in Trans- fusion Medicine was performed using the UHT SNP Stream System. Nineteen samples were tested (Seven JK1, Seven JK1/JK2 and Five JK2). All samples showed a 100% correspondence with the UHT genotype results. 5. HPA-1a/HPA-1b PCR-RFLP Analysis.

The GP3A Exon 3 SNP detection method for HPA-1a/b genotyping (Appendix A) was compared to a subset of samples (n=18) using conventional PCR-RFLP analysis performed independently (FIG. 2). The results of the two assays were 100% concordant. In addition, a 217G nucleotide mutation 21 basepairs downstream of the GP3A SNP was present in sample 8. This mutation does not affect HPA-1b expression but is detected in the PCR-RFLP and is prone to interpretation error in the conventional PCR-RFLP assay. However, the sample was correctly genotyped as HPA-1b in our SNP assay. Accordingly, the present invention eliminates or minimizes error in HPA-1 results obtained since no confusing or confounding information results from the gel readings of the present invention. That is to say, the conventional RFLP detected the presence of an additional DNA fragment at ˜180 bp which represents a heterozygous HpA-1b/1b^(G217) allele and was correctly genotyped as HPA-1b/b by the present invention.

Example 2

However, it should be obvious to one skilled in the art that other methodologies and/or technologies for SNP identification could be used, providing that the novel DNA sequences disclosed above are also used. Other embodiments could include the following but without limitation to micro-arrays on glass slides or silica chips, the use of mass spectrometry, or oligo-ligation and extension techniques to detect the SNPs of interest.

A preferred method of the present invention relates to a method for the detection of blood group and HPA genotypes. The present invention also provides novel DNA sequences that are used as primers in a multiplex PCR format according to the present invention to amplify the genomic regions of interest. The present invention also provides novel combinations of DNA sequences that are used in said multiplex PCR format, and for novel DNA sequences that are used to detect blood group and platelet SNPs.

A preferred application of the present invention is in the blood collection and blood banking industry without limitation to red blood cell, platelet, and bone marrow donations. Canada has over 850,000 blood donations yearly, many from repeat donors. Eventually, after all repeat donors are tested (each donor is tested once), the analyses will be performed only on the blood of new donors. With over 29 blood group and 6 HPA systems encompassing over 250 antigens, the platform will find wide application in this industry.

The present invention additionally encompasses various embodiments relating to the detection of various SNPs for the determination of the various genotypes in a sample and for the determination of the corresponding phenotype. In a preferred embodiment, the present invention utilizes a platform to analyzes a cytidine-to-thymidine (C→T) single nucleotide polymorphism. The invention may also employ the multiplex detection of, but not limited to, C→A, A→T, and G→C SNPs, or any other nucleotide SNP related to blood group or platelet antigens.

The present invention may additionally include methods and products for the detection of clinically relevant blood group antigens whereby an antigen of interest is characterized by a genotypic identifier that exceeds a single nucleotide polymorphism. Specifically, the present invention may extend to include clinically relevant insertions or deletions or other nucleotide changes that characterize a blood group antigen of interest, such a multiple base pair insertion in an allele of interest. For example, a genotypic identifier corresponding to a blood group antigen of interest may be pre-characterized, suitable primers and probes for detection thereof may be prepared and a blood sample screened according to the teachings of the present invention.

The present invention provides DNA sequences corresponding to the PCR primer pairs optimized for multiplex use to identify blood group and platelet antigens simultaneously. Accordingly, the present invention provides the novel primer pair sequences listed in Table 1.

The present invention additionally provides novel DNA sequences used to identify the single nucleotide polymorphisms (SNPs) that represent underlying DNA blood group and platelet antigens. Accordingly, the present invention provides the novel extension probes listed in Table 2.

The present invention provides a method of a combined analysis of blood group and HPA SNP analyses.

The present invention advantageously utilizes PCR, the variant and unique SNPs for the variant alleles that infer blood group phenotypes, and single base extension and detection chemistry as a foundation for the novel products and methods of the present invention. Accordingly, the present invention provides a high throughput, multiplexed, DNA-based method of blood group genotyping that replaces the current manual, semi-automated and automated serological screening process used to determine blood group phenotypes.

Accordingly, the present invention provides a method for the identification of rare blood group genotypes due to the suite of SNPs as described above, and in some instances replaces the current state of the art in which most rare blood group genotypes are identified serendipitously (propositus and their relatives) and enabling significant advances over current serological technologies. For example, by analyzing the SNP for the RhC allele in Rh negative blood, we can identify RhC homozygotes and thereby, the rare RhD-negative and Rhc-negative blood.

The present invention additionally provides a method of use in tissue compatibility matching for the purposes, without limitation, of organ transplantation, bone marrow transplantation and blood transfusion related to blood group and platelet antigens.

The present invention additionally provides novel components and constituents that are beneficial for the analyses relating to the present invention. More specifically, the group of currently developed SNPs representing a ‘suite’, or the presently known set of SNPs that relate to clinically important blood group and HPA genotypes for red blood cell and platelet antigens, respectively are provided. The present invention is not limited to the presently listed SNPs, but is understood to comprise all blood group and platelet antigen, and preferably HPA SNPs that may be analyzed in accordance with the teachings of the present invention and using the products, protocols and methods of the present invention.

The present invention also provides the DNA primer sequences optimized for use in a multiplex PCR format.

The present invention also provides novel DNA probe sequences used to detect the SNPs of interest.

The present invention provides a method for the simultaneous detection of a plurality of blood group SNPs. More specifically, the present invention provides a method for the simultaneous detection of at least 19 blood group SNPs; RHD (2), RHC/c, RHE/e, S/s, Duffy (a/b), Kidd (a/b), Diego (a/b), Kell K1/K2, Kell K3/K4, and HPA-1a/b simultaneously. The method of the present invention provides (1) DNA sequences corresponding to the PCR primer pairs optimized for multiplex use to identify a plurality of blood group and platelet antigens simultaneously; (2) Novel DNA sequences used to identify the single nucleotide polymorphisms (SNPs) that represent underlying DNA blood group and platelet antigens; and (3) The combination of SNP analyses including blood group and platelet antigens.

To support and validate the teachings of the present invention various experimental tests have been completed and analyzed. Numerous validating experimental data has been recorded, however, for the purpose of simplicity the following example is provided. Each step in the validating experiment is noted below:

(1) Ultra high throughput (UHT) Multiplex SNP analyses on 372 unrelated blood donor specimens for RHD (2), RHC/c, RHE/e, S/s, FY1/FY2 (2), JK1/JK2, DI1/D12, KEL1/KEL2, KEL3/KEL4, and HPA-1A/B genotypes and corresponding phenotypes was examined, and data was recorded (please refer to Appendix A for the raw data accumulated, and Table 5 for a Summary of the results obtained). (2) Manual PCR-RFLP analyses was performed on some of the 372 specimens for some of the blood group SNPs to for comparison to the results obtained in Step (1). (3) Serological analyses was also performed on some of the 372 specimens for each of the blood group and HPA SNPs using Health Canada regulated reagents performed by licensed medical technologists in a provincially licensed laboratory. (4) Serological analyses was also performed on some of the 372 specimens for each of the blood group and platelet antigens by unlicensed research technologists using Health Canada regulated reagents and methodologies in an unlicensed laboratory.

The results obtained from the above validating experimental data is provided below by way of supportive Figures and Tables.

1. SNP Platform Data Generation.

The robotic platform produces fluorescence for each sample which are presented in ‘scatter plots’ (as illustrated in FIG. 1) for the operator to review. Sample genotype results are shown for each blood group SNP and are graphed using logarithmic and XY scatter plots (upper right). Green, orange or blue sample designations represent CC, TC and TT genotype calls respectively. No fluorescence represents an assay failure (FL) for that sample.

2. SNP Data Manipulation and Analysis.

The SNP results of a scatter plot are electronically exported to a spreadsheet and examined for total sample failure and individual SNP failure rates. Twelve SNP results for 372 DNA samples are summarized in Table 3 with sample failure rates (shown on the right) and individual SNP success rates (shown at the bottom). Three hundred and fifty seven of 372 samples (96%) had results for at least one SNP. Individual SNP results ranged from 80% to 100%; only one SNP result success rate was <98%. Individual SNP failures do not affect the results of a sample for other SNPs that do not fail.

3. SNP Allele Frequency Analysis.

The SNP results where then compared with published phenotype frequencies for Caucasians and Blacks and are summarized in Table 5 above. The data shows that the allele frequencies are consistent with the accepted published frequencies for Caucasians and Blacks.

3.1 SNP Allele Result Compared to the Serological Result.

RhD status was compared between the serological result and the SNP analysis for RHD exon 4, and 9 (RHD Exon 4, RHD Exon 9, respectively). Table 4 summarizes the comparison. 287 of 291 (98.6%) RhD positive units and 55 of 66 (83.3%) RhD negative units were identified correctly using the UHT SNP platform.

3.2 SNP Analysis Compared to Manual PCR-RFLP.

Some of the UHT SNP genotype results were compared with manual PCR-RFLP analysis performed independently. The results show 100% concordance. A representative PCR-RFLP is shown in FIG. 3.

The genotyping technology provided in the present invention queries and analyzes SNPs using single base-pair primer extension. In brief, the genomic region surrounding the SNP of interest is amplified and used as a template for the ensuing hybridization and single nucleotide extension of the SNP specific extension primer. The extension primer is designed to hybridize adjacent to the polymorphic nucleotide(s) and enables us to query bi-allelic polymorphisms, small insertions, deletions or inversions. The 5′ extension primer tags are hybridized to the complementary DNA sequence on micro-arrayed plates and incorporation of Bidopy- and Tamra-labeled ddNTPs are detected by laser-microplate fluorescence for each individual blood group and HPA SNP. Individual sample genotypes are generated through automated imaging and analysis software as shown in the genotype scatter plots of FIG. 1.

The embodiment(s) of the invention described above is(are) intended to be exemplary only. The scope of the invention is therefore intended to be limited solely by the scope of the appended claims.

APPENDIX A Genotype Results for updated 12 SNP CBS Panel Sample Pass Sample ID RHD4 RHD7 RHD9 RHCE2 RHCE5 KEL6 KEL8 DI18 FYP FY2 GP3A JK9 FL Rate BB24401 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24402 TT FL CC CC TC CC CC CC TT CC TC CC 1 91.7% BB24407 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24408 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24409 TC TT TC TC TC CC CC CC TT CC CC TC 0 100.0% BB24410 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24415 TC TT TC TC FL CC CC CC TT TT TT TC 1 91.7% BB24416 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24417 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24420 TC TT TC TC CC CC CC CC TT CC TT CC 0 100.0% BB24421 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24422 TC TT TC FL TC CC CC CC TT TC TC TC 1 91.7% BB24423 TC TT TC TC TC TC CC CC TT TC TT TT 0 100.0% BB24424 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24425 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24426 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24427 TC TT TC TC TC TC CC CC TT TC TC CC 0 100.0% BB24428 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24429 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24430 TC TT TC TC TC TC CC CC TT TT TC CC 0 100.0% BB24431 TC TT TC TC CC TC CC CC TT TT TC TT 0 100.0% BB24432 TC TT TC TC CC CC CC CC TT TT TC TT 0 100.0% BB24433 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24434 TC TT TC TC CC CC CC CC TT CC TT TT 0 100.0% BB24435 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24436 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24437 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24438 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24439 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24440 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24444 TC TT TC TC CC TC CC CC TT TC TT TC 0 100.0% BB24448 TT FL CC CC FL CC CC CC TT TC TT CC 2 83.3% BB24461 TC TT TC TC CC CC CC CC TT TC TC CC 0 100.0% BB24462 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24463 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24464 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24465 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24466 TC TT TC FL TC CC CC CC TT TT TC TC 1 91.7% BB24467 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24468 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24469 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24470 TT FL CC CC TC CC CC CC TT TT CC CC 1 91.7% BB24471 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24472 TC TT TC TC TC CC CC CC TT TC TC CC 0 100.0% BB24473 TC TT TC TC TC TC CC CC TT TT TT CC 0 100.0% BB24474 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24475 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24476 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24477 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24478 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24479 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24480 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24481 TC TT TC TC CC CC CC CC TT CC TT TT 0 100.0% BB24482 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24483 TT FL CC CC TC CC CC CC TT TT TT TC 1 91.7% BB24484 TC TT TC TC TC TC CC CC TT CC TC TC 0 100.0% BB24485 TT FL CC FL TC TC CC CC TT TC TC TC 2 83.3% BB24486 TT FL CC CC TC CC CC CC TT TT TT TT 1 91.7% BB24487 TC TT TC TC TC CC CC CC TT TT TC CC 0 100.0% BB24488 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24489 TC TT TC TC TC CC CC CC TT TT TC CC 0 100.0% BB24491 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24492 TT FL CC CC TC CC CC CC TT CC TT CC 1 91.7% BB24493 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24494 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24495 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24496 TC TT TC TC TC CC CC CC TT TT TT CC 0 100.0% BB24497 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24499 TC TT TC TC TC CC CC CC TT TT TT CC 0 100.0% BB24504 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24505 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24506 TC TT TC TC CC CC CC CC TT TT TC TC 0 100.0% BB24507 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24512 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24513 TC TT TC FL TC CC CC CC TT CC TT CC 1 91.7% BB24516 TC TT TC TC CC CC CC CC TT TT TC TT 0 100.0% BB24517 TT FL CC CC TC TC CC CC TT TC TT TT 1 91.7% BB24518 TC TT TC TC TC CC CC CC TT CC TC TC 0 100.0% BB24519 TC TT TC TC CC CC CC CC TT TC TC CC 0 100.0% BB24522 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24523 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24524 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24525 TC TT TC FL TC CC CC CC TT TC TT TT 1 91.7% BB24526 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24527 TT FL CC CC TC CC CC CC TT CC TT TC 1 91.7% BB24528 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24529 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24530 TC TT TC TC CC CC CC CC TT TC TC CC 0 100.0% BB24531 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24532 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24533 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24534 TC TT TC FL TC TC CC CC TT TT TT TT 1 91.7% BB24535 TC TT TC TC TC TC CC CC TT TC TT TC 0 100.0% BB24536 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24537 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24538 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24539 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24540 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24541 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24542 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24543 TC TT TC TC CC CC CC CC TT CC TT TT 0 100.0% BB24547 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24548 TT FL CC CC TC CC CC CC TT CC TT TC 1 91.7% BB24549 TT FL CC CC FL CC CC CC TT TC TT TC 2 83.3% BB24550 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24552 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24553 TC TT TC TC CC CC CC CC TT CC TT TT 0 100.0% BB24554 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24555 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24556 TC TT TC TC CC CC CC CC TT TT TT TT 0 100.0% BB24557 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24558 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24559 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24560 TT FL CC CC CC CC CC CC TT CC TT CC 1 91.7% BB24561 TC TT TC FL TC CC CC CC TT CC TC TC 1 91.7% BB24562 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24563 TT FL CC CC TC CC CC CC TT TT TC TC 1 91.7% BB24564 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24565 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24566 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24567 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24568 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24569 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24570 TC TT TC FL TC CC CC CC TT TT TC CC 1 91.7% BB24571 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24572 TT FL CC CC TC TC CC CC TT TC TT TC 1 91.7% BB24573 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24574 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24575 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24576 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24577 TC TT TC TC TC TC CC CC TT TT TT CC 0 100.0% BB24578 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24579 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24580 TT FL FL CC TC CC CC CC TT TC TT CC 2 83.3% BB24581 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24586 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24587 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24594 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24600 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24601 TC TT TC FL TC CC CC CC TC TC TT CC 1 91.7% BB24602 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24603 TC TT TC FL TC CC CC CC TT TC TT TT 1 91.7% BB24604 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24605 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24606 TC TT TC TC CC CC CC CC TT TT TT CC 0 100.0% BB24607 TC TT TC TC TC CC CC CC TC TT TC CC 0 100.0% BB24608 TC TT TC TC CC CC CC CC TT TT TT CC 0 100.0% BB24609 TC TT TC FL TC CC CC CC TT CC TC CC 1 91.7% BB24610 TT FL CC CC TC CC CC CC TT TC TT TT 1 91.7% BB24611 TC TT TC TC CC CC CC CC TT CC TT TT 0 100.0% BB24612 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24613 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24614 TC TT TC TC CC CC CC CC TT TT TT TT 0 100.0% BB24615 TC TT TC TC CC TC CC CC TT TT TT CC 0 100.0% BB24616 TC TT TC TC TC TC CC CC TT TC TT TT 0 100.0% BB24617 TC TT TC FL TC CC CC CC TT TC TT TT 1 91.7% BB24618 TC TT TC TC TC CC CC TC TT TC TC TC 0 100.0% BB24619 TC TT TC FL FL CC CC CC TT TT TT TC 2 83.3% BB24620 TC TT TC FL FL CC CC CC TT CC TT TC 2 83.3% BB24621 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24622 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24623 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24624 TT FL CC CC TC CC CC CC TT TT TC TC 1 91.7% BB24625 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24626 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24627 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24628 TC TT TC TC TC TC CC CC TT TC TC TC 0 100.0% BB24629 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24630 TC TT TC TC CC CC CC CC TT TT TT TT 0 100.0% BB24631 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24632 TC TT TC TC TC CC CC CC TC TT TT TC 0 100.0% BB24633 TC TT TC FL TC CC CC CC TT TT TC TC 1 91.7% BB24634 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24635 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24636 TC TT TC FL TC CC CC CC TT CC TT CC 1 91.7% BB24637 TC TT TC FL TC CC CC CC TT TT TT TT 1 91.7% BB24638 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24639 TT FL CC CC TC CC CC CC TT CC TT TC 1 91.7% BB24640 TC TT TC TC CC CC CC CC TT TT TT CC 0 100.0% BB24641 TT FL CC CC TC CC CC CC TT TT TT TT 1 91.7% BB24642 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24643 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24644 TT FL FL FL TC FL CC CC TT TC TT CC 4 66.7% BB24645 TC TT TC TC CC CC CC CC TT TT TT CC 0 100.0% BB24646 TT FL CC CC TC CC CC CC TT TT TT TT 1 91.7% BB24647 TC TT TC TC TC TC CC CC TT TC TT TC 0 100.0% BB24648 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24649 TC TT TC TC TC CC CC CC TT TT TC TT 0 100.0% BB24650 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24651 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24652 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24653 TC TT TC TC TC CC CC CC TT CC TC TT 0 100.0% BB24654 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24655 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24656 TT FL CC CC TC CC CC CC TT TT TC TC 1 91.7% BB24657 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24658 TT FL CC CC TC CC CC CC TT CC TT TC 1 91.7% BB24659 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24660 TT FL CC CC TC CC CC CC TT TT TT TC 1 91.7% BB24661 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24662 TC TT TC TC TC CC CC CC TT TC TC TT 0 100.0% BB24663 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24664 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24665 TT FL FL CC TC FL CC FL TT TC CC TC 4 66.7% BB24666 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24667 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24668 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24669 TC TT TC TC CC TC CC CC TT CC TC CC 0 100.0% BB24670 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24672 TC TT TC TC TC CC CC CC TT TC TC CC 0 100.0% BB24673 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24674 TC TT TC TC CC CC CC CC TT TT TC CC 0 100.0% BB24675 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24676 TC TT TC FL TC CC CC CC TT TC TC TC 1 91.7% BB24678 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24679 TC FL TC TC TC CC CC CC TT CC TT TT 1 91.7% BB24680 TC TT TC TC CC CC CC CC TT TT TT TT 0 100.0% BB24681 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24682 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24683 TT FL CC CC TC CC CC CC TT TC TC TT 1 91.7% BB24684 TC TT TC TC CC CC CC CC TT TC TC TC 0 100.0% BB24685 TC TT TC TC CC CC CC CC CC TT TT CC 0 100.0% BB24686 TC TT TC TC CC CC CC CC TT CC TC TC 0 100.0% BB24687 TC TT TC TC TC CC CC CC TT CC TC TT 0 100.0% BB24688 TT FL CC CC TC CC CC CC TT TC TT TT 1 91.7% BB24689 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24690 TC TT TC TC CC CC CC CC TT CC TC CC 0 100.0% BB24691 TC TT TC TC CC CC CC CC TT TT CC CC 0 100.0% BB24692 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24693 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24694 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24695 TT FL CC CC TC CC CC CC TT TT TT CC 1 91.7% BB24696 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24697 TC TT TC TC TC TC CC CC TT TT TT TT 0 100.0% BB24698 TC TT TC TC CC CC CC CC TT TT TT TC 0 100.0% BB24699 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24700 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24701 TT FL CC CC TC CC CC CC TT TT TT CC 1 91.7% BB24702 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24703 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24704 TC TT TC FL TC CC CC CC TT CC TT TC 1 91.7% BB24705 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24706 TT FL FL CC TC CC CC CC TT CC TT TC 2 83.3% BB24707 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24708 TC TT TC FL TC CC CC CC TT TC TC TC 1 91.7% BB24709 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24710 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24711 TC FL TC TC TC TC CC CC TT TC TT CC 1 91.7% BB24712 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24713 TC FL TC FL TC CC CC CC TT CC TT TT 2 83.3% BB24714 TT FL CC CC TC CC CC CC TT TC TT TT 1 91.7% BB24715 TT FL CC CC TC CC CC CC TT TC TT TC 1 91.7% BB24716 TC TT TC TC CC CC CC CC TT TC TC CC 0 100.0% BB24717 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24718 TC TT TC TC TC CC CC CC TT TC TC TT 0 100.0% BB24719 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24720 TC TT TC TC CC CC CC CC TT TC TT TC 0 100.0% BB24721 TC TT TC TC TC CC CC CC CC TT TT TC 0 100.0% BB24722 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24723 TC TT TC TC CC CC CC CC TT TT TC CC 0 100.0% BB24724 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24725 TT FL CC CC TC CC CC CC TT CC TT CC 1 91.7% BB24726 TC TT TC TC TC CC CC CC TT TT TC TT 0 100.0% BB24727 TC TT TC TC CC CC CC CC TT TT TC TC 0 100.0% BB24728 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24729 TC TT TC FL TC CC CC CC TT CC TT CC 1 91.7% BB24730 TC FL TC TC CC CC CC CC TT TT TC TT 1 91.7% BB24731 TC TT TC TC CC CC CC CC TT TT TC TC 0 100.0% BB24732 TT FL CC CC TC CC CC CC TT TT TT CC 1 91.7% BB24733 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24734 TC TT TC TC CC TC CC CC TT TC TT TC 0 100.0% BB24735 TT FL CC CC TC CC CC CC TT TT TT TT 1 91.7% BB24736 TC TT TC TC TC TC CC CC TT TC TT TT 0 100.0% BB24737 TC TT TC FL TC CC CC CC TT TT TT TT 1 91.7% BB24738 TT FL CC CC TC CC CC CC TT TC TC CC 1 91.7% BB24739 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24740 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24741 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24742 TC TT TC TC TC CC CC CC TC TT TT TT 0 100.0% BB24743 TC TT TC TC TC CC CC CC TT TT TT TT 0 100.0% BB24744 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24745 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24746 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24747 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24748 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24749 TT FL CC CC CC CC CC CC TT TC TT TT 1 91.7% BB24750 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24751 TC TT TC TC TC CC CC CC TC TC TT TC 0 100.0% BB24752 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24753 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24754 TC TT TC TC CC CC CC CC TT TC TC FL 1 91.7% BB24755 TT FL CC CC TC CC CC CC TT TC TT TT 1 91.7% BB24756 TC FL TC TC CC CC CC CC TT TT TC TT 1 91.7% BB24757 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24758 TC TT TC TC TC CC CC CC TT CC TC TC 0 100.0% BB24759 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24760 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24761 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24762 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24763 TC TT TC TC FL TC CC CC TT CC FL TC 2 83.3% BB24764 TT FL CC TC TC CC TC CC TT TT TT CC 1 91.7% BB24765 TC FL TC TC CC CC CC CC TT TT TT CC 1 91.7% BB24766 TC TT TC FL TC CC CC CC TT TC TT TC 1 91.7% BB24767 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24768 TC TT TC FL TC CC CC CC TT TT TT TC 1 91.7% BB24769 TC TT TC TC CC CC CC CC TT CC TT CC 0 100.0% BB24770 TT FL CC CC CC CC CC CC TT CC TT CC 1 91.7% BB24771 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24772 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24773 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24774 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24775 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24776 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24777 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24778 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24779 TC FL TC TC CC CC FL CC TT TT TT TT 2 83.3% BB24780 TC TT TC TC TC CC CC CC TT TC TC CC 0 100.0% BB24781 TC TT TC TC TC CC CC CC TT TC TC CC 0 100.0% BB24782 TC TT TC TC TC CC CC CC TT CC TT CC 0 100.0% BB24783 TT FL CC CC TC CC CC CC TT TC TC TC 1 91.7% BB24784 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24785 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24786 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24787 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24788 TC TT TC TC CC CC CC CC TT TT TC TC 0 100.0% BB24789 TC TT TC TC TC TC CC CC TT TT TT TT 0 100.0% BB24790 TT FL CC CC TC CC CC CC TC TT TT TC 1 91.7% BB24791 TT FL CC FL TC CC FL CC TT TC TT CC 3 75.0% BB24792 TC TT TC TC CC TC CC CC TT TT TT TC 0 100.0% BB24793 TT FL CC CC FL CC CC CC TT TT TT TC 2 83.3% BB24794 TC TT TC TC CC CC CC CC TT CC TC CC 0 100.0% BB24795 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24796 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24797 TC TT TC TC TC CC CC TC TT TT TT TT 0 100.0% BB24798 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24799 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24800 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24801 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24803 TC TT TC TC CC CC CC CC TT CC TT FL 1 91.7% BB24804 TT FL CC CC TC CC CC CC TT TC TT CC 1 91.7% BB24805 TC TT TC TC CC CC CC CC TT TT TT TT 0 100.0% BB24806 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24807 TC TT TC FL TC CC CC CC TC TT TT CC 1 91.7% BB24808 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24809 TC TT TC TC TC CC CC CC TT TC TC CC 0 100.0% BB24810 TC TT TC TC CC CC CC CC TT TC TT TT 0 100.0% BB24811 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24812 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24815 TC TT TC TC TC CC CC CC TT TT TC TC 0 100.0% BB24817 TC TT TC TC TC CC CC CC TT CC TT TT 0 100.0% BB24818 TC TT TC TC TC CC CC CC TT TC TT CC 0 100.0% BB24819 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24820 TC TT TC TC TC CC CC CC TT CC TC CC 0 100.0% BB24821 TT FL CC CC TC CC CC CC TT CC TT TT 1 91.7% BB24823 TC TT TC TC TC TC CC CC TT TC TC TT 0 100.0% BB24824 TC TT TC TC TC CC CC CC TT TT TC TT 0 100.0% BB24826 TC TT TC TC TC CC CC CC TT TC TT TC 0 100.0% BB24827 TT FL CC TC TC CC CC CC TT CC TT TT 1 91.7% BB24830 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24831 TC TT TC TC CC CC CC CC TT CC TT TC 0 100.0% BB24832 TT FL CC CC TC CC CC CC TT TT TT TC 1 91.7% BB24833 TC TT TC TC TC CC CC CC TT TC TC TC 0 100.0% BB24834 TC TT TC TC CC CC CC CC TT TC TT CC 0 100.0% BB24836 TC TT TC TC TC CC CC CC TT TT TT CC 0 100.0% BB24837 TC TT TC TC TC CC CC CC TT TC TT TT 0 100.0% BB24838 TC TT TC TC TC CC CC CC TT CC TT TC 0 100.0% BB24839 TC TT TC TC CC TC CC CC TT TC TT CC 0 100.0% BB24841 TC TT TC TC TC CC CC CC TT TT TT TC 0 100.0% BB24842 TC TT TC TC TC CC CC CC TT TC TC TT 0 100.0% BB24843 TT FL FL TC FL FL CC FL TT FL TC TC 6 50.0% BB24844 FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% BB24847 TC TT TC TC CC TC CC CC TT TT TT TT 0 100.0% Q1H2O FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% Q2H2O FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% Q3H2O FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% Q4H2O FL FL FL FL FL FL FL FL FL FL FL FL 12 0.0% RHD4 RHD7 RHD9 RHCE2 RHCE5 KEL6 KEL8 DI18 FYP FY2 GP3A JK9 Sample FL 15 86 20 54 23 18 17 17 15 16 16 17 Sample Pass 357 286 352 318 349 354 355 355 357 356 356 355 Call Rate 95.97% 76.88% 94.62% 85.48% 93.82% 95.16% 95.43% 95.43% 95.97% 95.70% 95.70% 95.43% Genotypes (N) XX (TT) 64 286 0 0 0 0 0 0 348 112 263 87 XY (TC) 293 0 293 260 246 28 1 2 7 155 89 178 YY (CC) 0 0 59 58 103 326 354 353 2 89 4 90 Allele Freq X (p) 58.96% 100.00%  41.62% 40.88% 35.24%  3.95%  0.14%  0.28% 98.46% 53.23% 86.38% 49.58% Y (q) 41.04%  0.00% 58.38% 59.12% 64.76% 96.05% 99.86% 99.72%  1.54% 46.77% 13.62% 50.42% 

1. A nucleic acid sequence according to any one of the sequences in Table
 1. 2. A nucleic acid sequence according to claim 1 for use as PCR primer pairs for multiplex SNP analysis of a plurality of SNPs simultaneously.
 3. A set of oligonucleotides comprising at least one or all the sense and antisense primer set of Table 1, or any combination thereof.
 4. A set of oligonucleotide primers comprising sense and antisense primer, wherein said oligonucleotide primer set is suitable for amplifying and detecting a plurality of blood group or HPA SNPs simultaneously in a single tube.
 5. A set of oligonucleotide primers, wherein said primer set is selected from one or more, or all, primers of Table 1 or any combination thereof.
 6. A nucleic acid sequence according to any one of the sequences in Table
 2. 7. A nucleic acid sequence according to claim 6 for use as extension probes for the identification of SNPs.
 8. A nucleic acid sequence according to claim 2 or 7, wherein said SNPs relate to blood group and platelet antigens.
 9. An oligonucleotide set that distinguishes between the blood group or HPA genotypes, wherein said oligonucleotide set specifically hybridizes to a selected SNP corresponding to a specific antigen genotype.
 10. An oligonucleotide set according to claim 9, wherein said set is selected from one or more, or all, primers of Table 1 or any combination thereof.
 11. An oligonucleotide set according to claim 10, wherein said at least one oligonucleotide hybridizes the HPA-1 GP3A SNP for the determination of the HPA genotype and corresponding phenotype.
 12. An oligonucleotide primer and probe set for analyzing a plurality of SNPs simultaneously, wherein said SNPs comprises one, more than one or any combination of SNPs selected from the group consisting of RhD RHD Exon 4 C/T; RhD RHD Exon 9 A/G; RhC/c RHCE Exon 2 T/C; RhE/e RHCE Exon 5 C/G; S/s GYPB Exon 4 T/C; K/k KEL Exon 6 T/C; Kp^(a)/Kp^(b) KEL Exon 8 T/C; FY/FY0 FY Promoter T/C; Fy^(a)/Fy^(b) FY Exon 2 G/A; Jk^(a)/Jk^(b) KIDD Exon 9 G/A; Di^(a)/Di^(b) DIEGO Exon 19 T/C; and HPA-1a/b GP3A Exon 3 T/C.
 13. An oligonucleotide primer and probe set for analyzing the SNPs of claim 12, wherein one, more than one or all of said primer set is selected from Table 1, and wherein one, more than one of all of said probe set is selected from Table 2, such that the selection of primer and probe combinations correspond to the SNP being analyzed.
 14. A method of simultaneously analyzing a plurality of antigens in a sample wherein said method comprises: (a) isolation and purification of genomic DNA from said sample; (b) multiplex PCR amplification of DNA regions encompassing all SNPs of interest, (c) the digestion of multiplex PCR amplified products with restriction enzymes; (d) identification of SNPs using single-base pair primer extension of the amplified DNA fragments using the probes of Table 2; (e) hybridization of extension products; and (f) analysis of SNP extension products to determine the SNP genotype.
 15. A method according to Claim 14, wherein said restriction enzymes are Exonuclease I and Shrimp alkaline phosphatase for the purpose of removing excess dNTPs and/or oligonucleotides.
 16. A method according to claim 14, wherein said extension products are hybridized to tag-arrayed microplate.
 17. A method according to claim 14, wherein the multiplex PCR amplification comprises amplification with the nucleotide primer and probes of any one of claims 1 to
 13. 18. A method according to claim 14, wherein a thermal cycler is used to carry out the single-pair primer extension.
 19. A method according to claim 14, wherein any machine or method capable of analyzing SNPs may be used.
 20. A method according to claim 14, wherein GenomeLab SNPstream (Beckman Coulter Inc.) is used to analyze SNP extension products.
 21. A method of claim 14, wherein said method is carried out in a single reaction tube or single well of a multiwell plate.
 22. A method of claim 14, wherein said method is automated.
 23. A method according to claim 14, wherein said antigens are red blood cell and platelet blood group antigens.
 24. A method according to claim 14, wherein said antigens are selected from the group consisting of ABO, Rh (D, C, c, E, e), MNS, P, Lutheran, Kell (K, k), Lewis, Duffy (Fy^(a), Fy^(b)), Kidd (Jk^(a), Jk^(b)) or any other antigen for which a SNP has been identified.
 25. A method for the simultaneous detection of the presence or absence of blood cell antigen SNPs simultaneously using one or more of the oligonucleotides of Table 1 and Table 2, or any corresponding combination thereof.
 26. A method according to any one of claims 14-25, wherein 12 blood group and HPA SNPs are analyzed in a single tube.
 27. A method according to claim 14, wherein the HPA-1 GP3A SNP is analyzed for the determination of HPA genotype and corresponding phenotype.
 28. A method for the identification of rare blood group genotypes, said method comprising identifying and analyzing the corresponding rare SNPs combinations thereof.
 29. A method of screening or analyzing a test sample for the presence or absence of blood group SNPs, wherein said analysis simultaneously screens a plurality of SNPs in a single reaction tube.
 30. A method according to claim 29, wherein said test sample is a human blood sample.
 31. A method according to claim 29, wherein said SNPs are selected from the SNPs of claim
 12. 32. A method according to claim 29, wherein said SNPs are any blood-group SNPs capable of distinguishing between blood group antigen phenotypes.
 33. A method according to claim 29, wherein said method comprises: (a) isolation and purification of genomic DNA from said sample; (b) multiplex PCR amplification of DNA regions encompassing all SNPs; (c) the digestion of multiplex PCR amplified products with restriction enzymes; (d) identification of SNPs using single-base pair primer extension of the amplified DNA fragments; (e) hybridization of extension products; and (f) analysis of SNP extension products to determine the SNP genotype.
 34. The use of the primer pairs of Table 1 in multiplex PCR and the probes of Table 2 for the analysis thereof.
 35. The use according to claim 34, wherein said multiplex PCR is carried out in a single reaction tube.
 36. The use according to claim 34, wherein said multiplex PCR is automated to simultaneously analyse blood group and platelet antigen (preferably HPA) SNPs.
 37. The use according to claim 34, wherein said SNP analysis results in antigen genotypes and corresponding phenotypes of a test sample.
 38. A method of antigen typing, preferably blood group antigen and platelet antigen, more preferably, human platelet antigen, using the primer pairs of Table 1, and analysis using the probes of Table
 2. 39. A method of claim 38, wherein said typing uses a multiplex PCR SNP analysis format, wherein said analysis is preferably automated.
 40. An oligonucleotide primer and probe set for analyzing a plurality of SNPs simultaneously, wherein said SNPs comprises one, more than one or any combination of SNPs selected from the group consisting of a SNP as represented in Tables 1, 1A or
 2. 41. A method of simultaneously analyzing a plurality of antigens in a sample wherein said method comprises: (a) isolation and purification of genomic DNA from said sample; (b) multiplex PCR amplification of DNA regions encompassing all SNPs of interest, (c) the digestion of multiplex PCR amplified products with restriction enzymes; (d) identification of SNPs using single-base pair primer extension of the amplified DNA fragments using probes corresponding to said SNPs of interest; (e) hybridization of extension products; and (f) analysis of SNP extension products to determine the SNP genotype.
 42. The method of claim 33, wherein said step of multiplex PCR amplification includes using the primer pairs of Table
 1. 43. The method of claim 33, wherein said step of identification of SNPs includes using the probes of Table
 2. 44. The method of claim 33, wherein said step of hybridization includes using the probes of Table
 2. 45. The method of claim 29, wherein said blood group SNP is a SNP of Table 1 or Table 1A. 